How to measure and evaluate binding affinities | eLife
WEBAug 6, 2020 — An equilibrium dissociation constant is the ratio of dissociation and binding rate constants (KD = ), and thus can be determined by directly measuring these rate constants. Because koff is concentration-independent, it is the easiest and most robust parameter to measure. describes the steps for this measurement.
Leadership team | About - eLife
WEBRandy Schekman. Founding Editor-in-Chief, HHMI, University of California, Berkeley, United States. eLife’s editors, early-career advisors, governing board, and executive staff work in concert to realise our mission.
The Natural History of Model Organisms: The secret lives of
WEBJun 4, 2015 — Drosophila: D. hydei (the larger, dark flies) and D. melanogaster and D. simulans (the smaller, lighter flies) interacting at a food source. Although there is little sexual dimorphism between males and females of the D. hydei species, males can be distinguished in the video because they approach other flies to court. In the D. melanogaster and D. simulans species, males are smaller than the ...
The MR-Base platform supports systematic causal inference ... - eLife
WEBMay 30, 2018 — Abstract. Results from genome-wide association studies (GWAS) can be used to infer causal relationships between phenotypes, using a strategy known as 2-sample Mendelian randomization (2SMR) and bypassing the need for individual-level data. However, 2SMR methods are evolving rapidly and GWAS results are often insufficiently curated, undermining ...
Sodium taurocholate cotransporting polypeptide is a functional …
WEBNov 13, 2012 — Liver diseases related to the human hepatitis B virus (HBV) kill about 1 million people every year, and more than 350 million people around the world are infected with the virus. Some 15 million of these people are also infected with the hepatitis D virus (HDV), which is a satellite virus of HBV, and this places them at an even higher risk of liver diseases, including cancer.
The ER–Golgi intermediate compartment is a key membrane
WEBAug 6, 2013 — A key step in autophagosome biogenesis is the generation of PE-lipidated LC3 by a ubiquitin-like conjugation system (Ichimura et al., 2000; Kabeya et al., 2000).The level of LC3 lipidation has long been a reliable measure of autophagy activity in vivo (Klionsky et al., 2012).In vitro LC3 lipidation has recently been reconstituted with synthetic liposomes, recombinant LC3 and other components ...
Whole-brain comparison of rodent and human brains using spatial …
WEBNov 7, 2022 — Here we examine the patterns of similarity between the mouse and human brain using a common space constructed from spatial gene expression data sets. We begin with an initial set of 2835 homologous genes. Subsequently, we present and evaluate a novel method for improving the resolution of mouse-human neuroanatomical correspondences using a ...
Visualizing cellular and tissue ultrastructure using Ten-fold Robust ...
WEBFeb 18, 2022 — Expansion microscopy (ExM) circumvents the diffraction limit of light microscopy by physically expanding the specimen four-fold in each dimension (Chen et al., 2015; Tillberg et al., 2016).Expansion is achieved by chemically anchoring proteins and other biomolecules directly to a hyper-swelling gel, followed by aggressive proteolysis to enable uniform swelling of the gel material.
Compact and highly active next-generation libraries for CRISPR
WEBSep 23, 2016 — Highly multiplexed pooled genetic screening methodologies have emerged as powerful and broadly accessible tools for systematically profiling gene function at the scale of mammalian genomes (Paddison et al., 2004).Recently, a number of pooled screening platforms have been developed that utilize the bacterial CRISPR (Clustered Regularly Interspaced Palindromic Repeats)-associated nuclease Cas9 ...
Impact of a human gut microbe on Vibrio cholerae host …
WEBMar 28, 2022 — In this work, the authors study the previously reported positive association between the presence of the gut bacterium Paracoccus aminovorans and Vibrio cholerae during infection. They describe and image dual-species biofilm formed in vitro as well as enhanced V. cholerae gut colonization in the presence of P. aminovorans in a neonatal mouse model. . Collectively, the authors conclude that P ...